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Amersham Life Sciences Inc colloidal gold particles
Colloidal Gold Particles, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/colloidal+gold+particles/anti+gold+particles/pm41854930-58-10-13
Average 86 stars, based on 1 article reviews
colloidal gold particles - by Bioz Stars, 2026-10
86/100 stars

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Incubation:

Article Title: Expression of integrin receptors and their role in adhesion, spreading and migration of normal human melanocytes.
Article Snippet: Melanocytes are neural crest-derived cells endowed with defined morphological and biochemical markers, and are mainly located within the basal layer of the epidermis.. Melanocytes lie on the epidermal basement membrane where they closely interact with surrounding basal keratinocytes to form a functional structure defined as the epidermal-melanin unit (Weiss and Greep, 1977).. Melanocytes protect keratinocytes against ultraviolet damage (such as photocarcinogenesis) through the synthesis of the melanin pigment and the transfer of melanosomes to adjacent basal keratinocytes by multiple long dendritic processes (Weiss and Greep, 1977).

Article Title: Selective localization of Bcl-2 to the inner mitochondrial and smooth endoplasmic reticulum membranes in mammalian cells.
Article Snippet: .. Sections on grids were treated with 1% BSA in PBS, incubated with anti-Bcl-2 or -mitochondrial marker proteins overnight and with respective secondary antibodies coupled to 5 nm colloidal gold particles (Amersham) for 1 h. For double labeling, some grids were also incubated with both monoclonal anti-Bcl-2 and polyclonal anti-mitochondrial marker proteins, and then with anti-mouse IgG coupled with 15 nm gold particles and anti-rabbit IgG coupled with 5 nm gold particles (Amersham). ..

Article Title: Immunolocalization of the Na(+)-K(+)-2Cl(-) cotransporter in peripheral nervous tissue of vertebrates.
Article Snippet: ÐEf ̄ux of Cl through GABAA-gated anion channels depolarizes the cell bodies and intraspinal terminals of sensory neurons, and contributes to the generation of presynaptic inhibition in the spinal cord.. Active accumulation of Cl inside sensory neurons occurs through an Na±K±2Cl cotransport system that generates and maintains the electrochemical gradient for this outward Cl current.. We studied the immunolocalization of the Na±K±2Cl cotransporter protein using a monoclonal antibody (T4) against a conserved epitope in the C-terminus of the molecule.

Article Title: Erythema multiforme associated human autoantibodies against desmoplakin I and II: biochemical characterization and passive transfer studies into newborn mice.
Article Snippet: .. Briefly, for indirect immunogold labeling ultrathin cryosections of normal human skin were quenched in PBS/50 mM glycine (BioRad Laboratories, Richmond, CA)/10% fetal calf serum (GIBCO-BRL, Gaithersburg, MD), consecutively incubated with affinity-purified peptidespecific human or anti-peptide rabbit IgG, washed, and further exposed to goat anti-human or goat anti-rabbit IgG conjugated to 10 nm colloidal gold particles (Amersham, Bucks, U.K.). ..

Marker:

Article Title: Selective localization of Bcl-2 to the inner mitochondrial and smooth endoplasmic reticulum membranes in mammalian cells.
Article Snippet: .. Sections on grids were treated with 1% BSA in PBS, incubated with anti-Bcl-2 or -mitochondrial marker proteins overnight and with respective secondary antibodies coupled to 5 nm colloidal gold particles (Amersham) for 1 h. For double labeling, some grids were also incubated with both monoclonal anti-Bcl-2 and polyclonal anti-mitochondrial marker proteins, and then with anti-mouse IgG coupled with 15 nm gold particles and anti-rabbit IgG coupled with 5 nm gold particles (Amersham). ..

Labeling:

Article Title: Selective localization of Bcl-2 to the inner mitochondrial and smooth endoplasmic reticulum membranes in mammalian cells.
Article Snippet: .. Sections on grids were treated with 1% BSA in PBS, incubated with anti-Bcl-2 or -mitochondrial marker proteins overnight and with respective secondary antibodies coupled to 5 nm colloidal gold particles (Amersham) for 1 h. For double labeling, some grids were also incubated with both monoclonal anti-Bcl-2 and polyclonal anti-mitochondrial marker proteins, and then with anti-mouse IgG coupled with 15 nm gold particles and anti-rabbit IgG coupled with 5 nm gold particles (Amersham). ..

Article Title: Erythema multiforme associated human autoantibodies against desmoplakin I and II: biochemical characterization and passive transfer studies into newborn mice.
Article Snippet: .. Briefly, for indirect immunogold labeling ultrathin cryosections of normal human skin were quenched in PBS/50 mM glycine (BioRad Laboratories, Richmond, CA)/10% fetal calf serum (GIBCO-BRL, Gaithersburg, MD), consecutively incubated with affinity-purified peptidespecific human or anti-peptide rabbit IgG, washed, and further exposed to goat anti-human or goat anti-rabbit IgG conjugated to 10 nm colloidal gold particles (Amersham, Bucks, U.K.). ..

Affinity Purification:

Article Title: Erythema multiforme associated human autoantibodies against desmoplakin I and II: biochemical characterization and passive transfer studies into newborn mice.
Article Snippet: .. Briefly, for indirect immunogold labeling ultrathin cryosections of normal human skin were quenched in PBS/50 mM glycine (BioRad Laboratories, Richmond, CA)/10% fetal calf serum (GIBCO-BRL, Gaithersburg, MD), consecutively incubated with affinity-purified peptidespecific human or anti-peptide rabbit IgG, washed, and further exposed to goat anti-human or goat anti-rabbit IgG conjugated to 10 nm colloidal gold particles (Amersham, Bucks, U.K.). ..



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